Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva Tricorn Columns
Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.
| Includes | Glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock and filter holder |
|---|---|
| Product Type | Tricorn Columns (Empty) |
| Quantity | 1 Ea. |
| Material | Borosilicate glass |
| For Use With (Application) | High resolution liquid chromatography of biomolecules performed at medium to high pressures |
| Support Type | Polypropylene filter |
Cytiva Dextrin Sepharose™ High Performance Media
Provide convenient affinity purification of recombinant proteins with maltose binding protein (MBP) tags
| Particle Size | 34 μm |
|---|---|
| dimMatrix | Affinity Resin |
| Material | Dextrin Sepharose |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
|---|---|
| Diameter (English) Inner | 0.62 in. |
| Bed Volume | 5 mL |
| Description | GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| For Use With (Application) | For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract |
| Particle Size | 90 μm |
| Ligand Type | Glutathione and 10 carbon linker arm |
| Flow Rate | <20 mL/min. |
| Type | Pre-packed Column |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| Support Type | 4% Highly Cross-linked Agarose |
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
| Particle Size | 34μm |
|---|---|
| Buffer | 6M Guanidine Hydrochloride, 8M Urea |
| Ligand Type | Iminodiacetic Acid |
| Height (Metric) | 25mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 13 |
| Product Type | HiTrap chelating HP column |
| Column Type | Affinity Column |
| For Use With (Application) | IMAC and subsequent purification of Polyhistidine tagged Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 3 bar |
| Height (English) | 0.98 in. |
| Column Type | Column Accesory |
|---|---|
| Quantity | 2 Pack |
| Material | Polypropylene |
Cytiva His MultiTrap™ 96-well Filter Plates
Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged proteins
| Type | 96-well Filter Plate |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC) |
| pH Range | 3 to 12 |
| Quantity | 4 x 96-well Filter Plates |
| Material | Polypropylene, Polyethylene |
Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
| Content And Storage | 4°C to 30°C |
|---|---|
| Support Type | 6% Highly Cross-linked Agarose |
| Particle Size | 75μm |
|---|---|
| Buffer | Aqueous Buffer |
| Ligand Type | N-benzyl-n-methyl Ethanolamine |
| Height (Metric) | 25mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | HiTrap Capto adhere column |
| Column Type | Affinity Column |
| Material | Polypropylene |
| For Use With (Application) | Easy screening and convenient process development |
| Support Type | Highly Cross-linked Agarose |
| Height (English) | 0.98 in. |
Thermo Scientific™ AminoLink™ Coupling Resin
Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.
| Stationary Phase | Aldehyde |
|---|---|
| Type | Resin |
| Target | Antibody, Proteins through Primary Amine |
| Content And Storage | Store at 4°C. |
| Format | Bottle |
| Column Type | Affinity, Agarose Resin |
| Product Line | AminoLink |
Thermo Scientific™ SulfoLink™ Coupling Resin
Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.
| Stationary Phase | Haloacetyl |
|---|---|
| Type | Resin |
| Content And Storage | Upon receipt store product in the dark at 4°C. Product is shipped at ambient temperature. |
| Format | Bottle |
| Column Type | Affinity, Agarose Resin |
| Sufficient For | 1 mg Peptide or 10 mg Antibody per mL of Resin |
| Product Line | SulfoLink |
Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
Used for method optimization and parameter screening
| Particle Size | 45 to 165μm |
|---|---|
| Height (Metric) | 100mm |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7 mL |
| Diameter (Metric) Inner | 7.7 mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1.5 bar |
| Height (English) | 3.93 in. |
Cytiva HiScreen™ Capto™ Q ImpRes and Capto™ SP ImpRes
High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands
| Particle Size | 36 to 44μm |
|---|---|
| Buffer | 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol |
| Flow Rate | 220 cm/hr. |
| Height (Metric) | 200mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 39.3 in. |
| Diameter (Metric) Inner | 1000mm |
| dimMatrix | Capto Q Chromatography Media |
| Quantity | 100 mL |
| For Use With (Application) | High-throughput intermediate purification and polishing steps of a wide range of biomolecules |
| Support Type | High-flow Agarose |
| Max. Pressure | <3 bar |
| Height (English) | 7.87 in. |
Cytiva HiScreen™ Sepharose™ Prepacked Fast Flow HIC Columns
Designed for method optimization and parameter screening
| Particle Size | 45 to 165μm |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7mL |
| pH Range | 3 to 13 |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 7.7mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| For Use With (Application) | Convenient process development optimization parameter screening |
| Max. Pressure | 1.5 bar |
| Height (English) | 3.93 in. |
Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns
Designed for method optimization and parameter screening
| Buffer | 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7mL |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 7.7mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| For Use With (Application) | Optimization and parameter screening, ion exchange chromato graphy |
| Max. Pressure | 1.5 bar |
| Particle Size | 45 to 165μm |
| Flow Rate | <450 cm/hr. |
| Support Type | 6% Cross-linked Agarose |
| Height (English) | 3.93 in. |
Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns
Designed for method optimization and parameter screening
| Buffer | 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7mL |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 7.7mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| For Use With (Application) | Optimization and parameter screening, ion exchange chromato graphy |
| Max. Pressure | 3 bar |
| Particle Size | 45 to 165μm |
| Flow Rate | <150 cm/hr. |
| Support Type | 6% Cross-linked Agarose |
| Height (English) | 3.93 in. |